Peptide Reconstitution Calculator
Draw this amount:10units
Concentration2.5 mg/mL
Volume to inject0.10 mL
Doses per vial20
How to Use This Peptide Calculator
1
Enter Peptide Amount
Input the total milligrams (mg) in your peptide vial. Common sizes: 2mg, 5mg, 10mg.
2
Add Water Volume
Enter how much bacteriostatic water (BAC water) you'll add. More water = easier to measure small doses.
3
Set Your Dose
Enter your desired dose in micrograms (mcg). The calculator shows exact syringe units to draw.
How MOTS-c Is Dosed
MOTS-c is a mitochondrial-derived peptide, a short peptide encoded within mitochondrial DNA that is studied for its role in metabolic regulation.
There is no published human pharmacokinetic data for injected MOTS-c. The only human trial ran on a different MOTS-c analog, not the peptide itself, so its data does not transfer and we do not present a half-life curve for MOTS-c in the app.
Unlike most research peptides that are dosed in micrograms, MOTS-c community protocols are dosed in milligrams, commonly around 2.5 to 10 mg per subcutaneous injection, two to three times per week, usually from a 10 mg vial. The calculator converts your chosen dose into exact units.
See also: MOTS-c: The Mitochondrial Peptide Linking Metabolism and Longevity
MOTS-c is a research compound. The doses shown are drawn from published trials or community protocols and are not clinically validated for self-administration. This tool is educational only and is not medical advice or a dosing recommendation.
MOTS-c Dosing Reference
| Peptide | Common Vial | Cited Dose | BAC Water | Units to Draw |
|---|---|---|---|---|
| MOTS-c | 10mg | 5mg | 2mL | 100 units |
| MOTS-c | 10mg | 2.5mg | 2mL | 50 units |
| Epithalon | 10mg | 5mg | 2mL | 100 units |
Community protocol figures, no clinical basis. For education only.
MOTS-c Dosage Chart: Reconstitution and Syringe Units
A mots-c dosage chart answers one question: once the vial is reconstituted, how many units on an insulin syringe correspond to a given amount. That is arithmetic, and it is identical for every peptide, so the chart below applies to MOTS-c exactly as it does to any other compound. It is not a protocol and it recommends no amount: what amount is appropriate for a person, if any, is a question for a qualified healthcare professional.
Reconstitution chart, using 2 mL of bacteriostatic water:
| Vial strength | Concentration | 1 syringe unit delivers | Units for 250 mcg | Units for 500 mcg | Units for 1 mg |
|---|---|---|---|---|---|
| 2 mg | 1 mg/mL | 10 mcg | 25 | 50 | 100 |
| 5 mg | 2.5 mg/mL | 25 mcg | 10 | 20 | 40 |
| 10 mg | 5 mg/mL | 50 mcg | 5 | 10 | 20 |
| 20 mg | 10 mg/mL | 100 mcg | 2.5 | 5 | 10 |
| 50 mg | 25 mg/mL | 250 mcg | 1 | 2 | 4 |
An insulin syringe unit is 0.01 mL, so one unit always delivers the concentration multiplied by 10 in micrograms. That single fact is what the whole chart is built on.
Using a different volume of water: the chart scales linearly. With 1 mL of bacteriostatic water the concentration doubles, so use half the units shown. With 3 mL it drops by a third, so use 1.5 times the units shown. The calculator at the top of this page does this for any combination, including volumes that are not round numbers.
Reference values only. Verify every calculation against your own vial before any handling, and remember that a reconstituted vial must be stored refrigerated.
The Math Behind Peptide Reconstitution
Concentration formula:
Concentration (mg/mL) = Peptide (mg) ÷ Water (mL)Units to draw:
Units = [Dose (mcg) ÷ 1000] ÷ Concentration × 100Example: BPC-157 250mcg dose
- 5mg vial + 2mL BAC water = 2.5 mg/mL
- 250mcg = 0.25mg
- 0.25mg ÷ 2.5 mg/mL = 0.10mL
- 0.10mL × 100 = 10 units on insulin syringe
Printable reconstitution chart, free
Get the reference chart by email (concentrations, µg per 10 units, formulas, storage) to keep next to your prep space.
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Discover Peptide LabUnderstanding Insulin Syringe Units
10 units = 0.10 mL
Quick Reference
- 100 units = 1 mL (full syringe)
- 50 units = 0.5 mL (half syringe)
- 10 units = 0.1 mL (1/10th syringe)
- 1 unit = 0.01 mL
Standard insulin syringes are U-100, meaning 100 units per mL. This calculator uses this standard.
Where to buy
Prices and stock are set by each vendor and can change. Always confirm third-party testing on the vendor site.
Frequently Asked Questions
Why is MOTS-c dosed in milligrams instead of micrograms?
Community MOTS-c protocols use milligram amounts (commonly 2.5 to 10 mg per injection), which is much larger than the microgram doses of most research peptides. Make sure your calculator dose is entered correctly, since our field uses micrograms (5 mg equals 5000 mcg).
What is the half-life of MOTS-c?
There is no published human pharmacokinetic data for injected MOTS-c, so a reliable half-life cannot be stated. The only human trial used a different MOTS-c analog, not the peptide itself.
Has MOTS-c been studied in humans?
Directly injected MOTS-c has not completed published human pharmacokinetic studies. Most of the evidence is preclinical, and a separate analog has been the subject of early clinical work.
What is bacteriostatic water (BAC water)?
Bacteriostatic water is sterile water containing 0.9% benzyl alcohol as a preservative. It prevents bacterial growth, allowing multi-use of reconstituted peptides for 4-6 weeks when refrigerated.
How much water should I add to my vial?
Common choices are 1mL, 2mL, or 3mL. More water makes small doses easier to measure accurately but means larger injection volumes. 2mL is a good balance for most peptides.
What does "units" mean on an insulin syringe?
Insulin syringes use "units" as measurements. Standard U-100 syringes have 100 units per 1mL, so 10 units = 0.10mL and 50 units = 0.50mL.
How long do reconstituted peptides last?
With bacteriostatic water, most peptides remain stable for 4-6 weeks when refrigerated at 2-8 degrees Celsius. With sterile water (no preservative), use within 24-48 hours.
Is this calculator free to use?
Yes, completely free with no signup required. For injection tracking, body maps and protocol management, see our full Peptide Lab app.
What is the MOTS-c dosage chart?
It is the conversion between a reconstituted MOTS-c vial and syringe units. Divide the vial strength in mg by the bacteriostatic water in mL to get the concentration, then multiply by 10 to get the micrograms delivered by one insulin unit. With a 5 mg vial in 2 mL, one unit delivers 25 mcg, so 250 mcg is 10 units. The chart on this page lays out the common vial strengths. It states no recommended amount.
How many units of MOTS-c is 250 mcg?
It depends entirely on how the vial was reconstituted, not on the peptide. With 2 mL of bacteriostatic water: a 2 mg vial gives 25 units, a 5 mg vial 10 units, a 10 mg vial 5 units. Halve those numbers if only 1 mL of water was used. Whether 250 mcg is an appropriate amount is a separate question, and one for a healthcare professional.